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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being documented but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged with the antibody.
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This guide outlines further more attainable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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